7K02
The crystal structure of a BAK dimer activated by detergent
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.320, 115.610, 44.530 |
Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
Resolution | 39.330 - 3.400 |
R-factor | 0.2983 |
Rwork | 0.296 |
R-free | 0.34270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uxm |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.330 | 3.522 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmeas | 0.057 | 1.222 |
Number of reflections | 11007 | 1083 |
<I/σ(I)> | 13.43 | 1.32 |
Completeness [%] | 99.6 | |
Redundancy | 3.9 | |
CC(1/2) | 0.999 | 0.484 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 291 | 50% (v/v) methane pentane diol (MPD) and 0.1 M imidazole pH 7.0 |