7JL8
Human PrimPol extending from the correct primer base C opposite the 8-oxoguanine lesion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-20 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.03324 |
Spacegroup name | P 1 |
Unit cell lengths | 50.689, 65.330, 72.001 |
Unit cell angles | 68.47, 85.67, 86.92 |
Refinement procedure
Resolution | 28.160 - 2.100 |
R-factor | 0.1883 |
Rwork | 0.187 |
R-free | 0.21330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l2x |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.162 | 2.160 |
High resolution limit [Å] | 2.099 | 2.100 |
Number of reflections | 48622 | 3825 |
<I/σ(I)> | 11.6 | |
Completeness [%] | 97.1 | 93.6 |
Redundancy | 3.4 | |
CC(1/2) | 0.998 | 0.780 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 225-250 mM CaCl2, 16-19% PEG 3350 |