7JK1

Human PrimPol inserting correct dCTP opposite the 8-oxoguanine lesion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979180
Spacegroup name	P 1
Unit cell lengths	52.558, 65.732, 75.179
Unit cell angles	69.66, 82.83, 88.54

Refinement procedure

Resolution	39.877 - 2.620
R-factor	0.2416
Rwork	0.239
R-free	0.28580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l2x
RMSD bond length	0.011
RMSD bond angle	0.681
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.950	2.740
High resolution limit [Å]	2.620	2.620
Rmerge	0.112	1.189
Number of reflections	27758	3360
<I/σ(I)>	6.1
Completeness [%]	98.7	98
Redundancy	2.1
CC(1/2)	0.988	0.375

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	225-250 mM CaCl2 16-19% PEG 3350