7JHP

Crystal structure of HRas in complex with the Ras-binding and cysteine-rich domains of CRaf-kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-08-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	94.487, 44.353, 74.395
Unit cell angles	90.00, 103.94, 90.00

Refinement procedure

Resolution	36.610 - 2.766
R-factor	0.2162
Rwork	0.211
R-free	0.26370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 4G0N & 1FAR
RMSD bond length	0.002
RMSD bond angle	0.442
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.610	2.865
High resolution limit [Å]	2.760	2.766
Rmerge	0.120	0.758
Rmeas	0.129	0.819
Rpim	0.048	0.308
Number of reflections	7159	369
<I/σ(I)>	15.63	2.08
Completeness [%]	91.5	49.27
Redundancy	7.2	6.9
CC(1/2)		0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 17% w/v PEG10000