7JHP
Crystal structure of HRas in complex with the Ras-binding and cysteine-rich domains of CRaf-kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-08-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 94.487, 44.353, 74.395 |
Unit cell angles | 90.00, 103.94, 90.00 |
Refinement procedure
Resolution | 36.610 - 2.766 |
R-factor | 0.2162 |
Rwork | 0.211 |
R-free | 0.26370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 4G0N & 1FAR |
RMSD bond length | 0.002 |
RMSD bond angle | 0.442 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.610 | 2.865 |
High resolution limit [Å] | 2.760 | 2.766 |
Rmerge | 0.120 | 0.758 |
Rmeas | 0.129 | 0.819 |
Rpim | 0.048 | 0.308 |
Number of reflections | 7159 | 369 |
<I/σ(I)> | 15.63 | 2.08 |
Completeness [%] | 91.5 | 49.27 |
Redundancy | 7.2 | 6.9 |
CC(1/2) | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 17% w/v PEG10000 |