7FB7

Crystal structure of human UHRF1 TTD in complex with 5-amino-2,4-dimethylpyridine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-05-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	147.120, 147.120, 110.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.140 - 1.450
R-factor	0.1584
Rwork	0.158
R-free	0.17330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yya
RMSD bond length	0.014
RMSD bond angle	1.493
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.140	1.480
High resolution limit [Å]	1.450	1.450
Number of reflections	157937	80771
<I/σ(I)>	21.2	2
Completeness [%]	100.0	100
Redundancy	9.7
CC(1/2)	1.000	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1 M HEPES (pH7.5), 45% v/v MPD, 0.2 M Ammonium acetate