7F7W
JAK2-JH2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.988 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.980, 57.770, 86.240 |
Unit cell angles | 90.00, 109.32, 90.00 |
Refinement procedure
Resolution | 57.546 - 1.830 |
R-factor | 0.2076 |
Rwork | 0.207 |
R-free | 0.23830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ut5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.000 |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.000 | 1.980 |
High resolution limit [Å] | 1.830 | 1.950 |
Number of reflections | 48992 | 4195 |
<I/σ(I)> | 14.5 | |
Completeness [%] | 94.4 | |
Redundancy | 6.4 | |
CC(1/2) | 0.999 | 0.968 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M Tris, pH 8.0 0.2M Sodium acetate, 12-20% PEG 4000 |