7F07
Autonomous VH domain that interacts with eIF4E at the Capped mRNA Binding site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.522, 81.819, 122.987 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.943 - 2.250 |
| Rwork | 0.214 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bea |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.311 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.996 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.120 | 0.755 |
| Number of reflections | 19226 | 2734 |
| <I/σ(I)> | 4.7 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.4 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M TRIS.HCl pH 8.5 25% (v/v) PEG 550 MME |
