7EZW
Cyclic Peptide that Interacts with the eIF4E Capped-mRNA Binding Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-29 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 63 |
| Unit cell lengths | 81.139, 81.139, 66.105 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.190 - 2.350 |
| Rwork | 0.205 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bea |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.143 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.194 | 2.470 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.114 | 0.711 |
| Number of reflections | 10511 | |
| <I/σ(I)> | 13.8 | 2.6 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 11.3 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Potassium chloride 20% (w/v) PEG 3350 |
