7EZJ

Crystal structure of p73 DNA binding domain complex bound with 1 bp and 2 bp spacer DNA response elements.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	200
Detector technology	CCD
Collection date	2011-02-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	139.697, 105.496, 155.504
Unit cell angles	90.00, 112.36, 90.00

Refinement procedure

Resolution	40.930 - 2.900
R-factor	0.2438
Rwork	0.241
R-free	0.30610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vd0
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.080	0.080
Number of reflections	91900	91900
<I/σ(I)>	19.5
Completeness [%]	99.5
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	100 mM MES, pH-6.0, 0.1 M Ammonium acetate, 12% w/v PEg20K