7EZC
Adenosine A2a receptor mutant-I92N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2020-07-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.230, 175.730, 112.710 |
| Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
| Resolution | 47.430 - 3.800 |
| R-factor | 0.289 |
| Rwork | 0.288 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qak |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.635 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.429 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 13682 | |
| <I/σ(I)> | 10.46 | 1.52 |
| Completeness [%] | 99.9 | |
| Redundancy | 43.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8.2 | 291 | 100 mM Tris pH 8.2, 30% PEG400 and 0.4 M (NH4)2SO4. |
