7EX8
Crystal structure of Ebinur Lake virus cap snatching endonuclease in complex with inhibitor 27
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.748, 70.692, 71.063 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.780 - 1.970 |
R-factor | 0.1967 |
Rwork | 0.195 |
R-free | 0.22690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xi5 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.040 |
High resolution limit [Å] | 1.970 | 4.240 | 1.970 |
Rmerge | 0.095 | 0.072 | 0.408 |
Rmeas | 0.107 | 0.082 | 0.476 |
Rpim | 0.048 | 0.038 | 0.239 |
Total number of observations | 70936 | ||
Number of reflections | 14633 | 1620 | 1232 |
<I/σ(I)> | 8.9 | ||
Completeness [%] | 96.8 | 98.1 | 82.7 |
Redundancy | 4.8 | 4.6 | 3.4 |
CC(1/2) | 0.993 | 0.892 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.7 | 289 | PEG 3350 |