7EVR

Crystal structure of hnRNP L RRM2 in complex with SETD2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	56.776, 38.789, 68.982
Unit cell angles	90.00, 100.31, 90.00

Refinement procedure

Resolution	39.780 - 1.800
R-factor	0.1602
Rwork	0.158
R-free	0.19790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zzy
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.127	0.073	0.466
Rmeas	0.141	0.081	0.516
Rpim	0.060	0.035	0.218
Total number of observations	142925
Number of reflections	27697	1465	1354
<I/σ(I)>	4.6
Completeness [%]	99.8	99.7	98.1
Redundancy	5.2	5.1	4.9
CC(1/2)		0.993	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.8M Potassium/Sodium phosphate, pH 7.5