7EQF

Crystal Structure of a Transcription Factor in complex with Ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.977945
Spacegroup name	C 1 2 1
Unit cell lengths	166.469, 53.556, 109.978
Unit cell angles	90.00, 95.20, 90.00

Refinement procedure

Resolution	47.730 - 2.910
R-factor	0.249
Rwork	0.248
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7eqe
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.730	3.080
High resolution limit [Å]	2.910	2.910
Number of reflections	40256	5721
<I/σ(I)>	8.69
Completeness [%]	96.8
Redundancy	3.42

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	HEPES-NaOH pH 7.5, polyethylene glycol 4000, isopropanol