7EQ2
Crystal structure of GDP-bound Rab1a-T75D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.977760 |
Spacegroup name | P 1 |
Unit cell lengths | 39.489, 50.598, 52.626 |
Unit cell angles | 76.94, 84.62, 86.27 |
Refinement procedure
Resolution | 25.116 - 1.551 |
R-factor | 0.161457428126 |
Rwork | 0.160 |
R-free | 0.18519 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fol |
RMSD bond length | 0.006 |
RMSD bond angle | 0.902 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.120 | 1.610 |
High resolution limit [Å] | 1.551 | 1.551 |
Number of reflections | 54416 | 5434 |
<I/σ(I)> | 42.71 | |
Completeness [%] | 95.2 | |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 291.15 | 40mM Lithium chloride, 20mM Magnesium chloride hexahydrate, 80mM NaCl, 2mM Hexammine cobalt(III) chloride, 40mM Sodium cacodylate trihydrate pH 5.5, 30% v/v (+/-)-2-Methyl-2,4-pentanediol |