Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-07-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.200, 211.450, 56.100 |
| Unit cell angles | 90.00, 96.96, 90.00 |
Refinement procedure
| Resolution | 55.687 - 3.000 |
| R-factor | 0.2257 |
| Rwork | 0.223 |
| R-free | 0.27120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6atd |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.687 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.153 | |
| Rmeas | 0.181 | 0.796 |
| Number of reflections | 36697 | 2771 |
| <I/σ(I)> | 5.82 | 1.65 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3.46 | 3.522 |
| CC(1/2) | 0.980 | 0.764 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2M sodium formate, 0.1 M Bicine ph 8.5, 15% w/v PEG 5000MME |
