7EDZ
Crystal Structure of human PPCS in complex with P-HoPan and AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.000, 100.000, 146.900 |
| Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
| Resolution | 36.720 - 1.950 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.20620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p9o |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.101 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.720 | 1.990 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 103396 | 7572 |
| <I/σ(I)> | 10.79 | |
| Completeness [%] | 98.2 | |
| Redundancy | 8.9 | |
| CC(1/2) | 0.996 | 0.928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 280 | 0.2 M Lithium Sulfate Monohydrate, 0.1 M Tris HCl, 30% PEG 4000 |
