7E9B
Structural basis of HLX10 PD-1 receptor recognition, a promising anti-PD-1 antibody clinical candidate for cancer immunotherapy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9751 |
Spacegroup name | P 1 |
Unit cell lengths | 38.985, 45.014, 80.890 |
Unit cell angles | 93.60, 102.16, 97.34 |
Refinement procedure
Resolution | 31.410 - 1.780 |
R-factor | 0.1716 |
Rwork | 0.170 |
R-free | 0.20980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zqk |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.414 | 1.840 |
High resolution limit [Å] | 1.779 | 1.779 |
Number of reflections | 48174 | 48174 |
<I/σ(I)> | 28.9 | |
Completeness [%] | 94.6 | |
Redundancy | 7.1 | |
CC(1/2) | 0.989 | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Tris pH 7.5 containing 23% (w/v) PEG 4000 |