7E8Z
Crystal structure of the human fat mass and obesity associated protein (FTO) in complex with SS81
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-24 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | H 3 |
| Unit cell lengths | 142.293, 142.293, 83.901 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.080 - 2.550 |
| R-factor | 0.1971 |
| Rwork | 0.195 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ie5 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.140 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.087 | 0.766 |
| Rmeas | 0.106 | 0.927 |
| Rpim | 0.060 | 0.514 |
| Number of reflections | 20599 | 1000 |
| <I/σ(I)> | 10.4 | 0.5 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.18 | 3.1 |
| CC(1/2) | 0.990 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 13.5% PEG3350, 100 mM trisodium citrate pH 5.6, 4% tert-butanol, 1 mM NADH |
