7E8M

Crystal structure of SARS-CoV-2 antibody P2C-1F11 with mutated RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9796
Spacegroup name	C 1 2 1
Unit cell lengths	195.844, 85.973, 57.872
Unit cell angles	90.00, 99.75, 90.00

Refinement procedure

Resolution	26.170 - 2.090
R-factor	0.1704
Rwork	0.169
R-free	0.19860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cdi
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.170	2.170
High resolution limit [Å]	2.090	2.090
Number of reflections	50710	3033
<I/σ(I)>	17.2
Completeness [%]	90.9
Redundancy	5.3
CC(1/2)	0.997	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M Ammonium sulfate, 0.1M Tris pH 8.5, 12%w/v PEG 8000