7E7E
The co-crystal structure of ACE2 with Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2021-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.779, 115.462, 224.668 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.840 - 3.800 |
| R-factor | 0.2781 |
| Rwork | 0.276 |
| R-free | 0.32700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r4l |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.870 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.415 | 1.739 |
| Number of reflections | 48694 | 2472 |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Sodium citrate tribasic dihydrate(pH 6.5),30% (v/v) PEG 550 |
