7E7C

Crystal structure of ENL YEATS domain T1 mutant in complex with histone H3 acetylation at K27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-02
Detector	MAR CCD 130 mm
Wavelength(s)	0.9779
Spacegroup name	P 21 21 2
Unit cell lengths	38.635, 118.065, 34.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.350 - 1.840
R-factor	0.2189
Rwork	0.215
R-free	0.25130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j9s
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.870
High resolution limit [Å]	1.840	4.990	1.840
Rmerge	0.129	0.081	0.816
Rmeas	0.138	0.088	0.852
Rpim	0.047	0.035	0.244
Total number of observations	141761
Number of reflections	14147	803	659
<I/σ(I)>	7.2
Completeness [%]	98.8	98	99.1
Redundancy	10	6.4	12.1
CC(1/2)		0.992	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	25% PEG 4000, 0.1M MES pH 6.5, 0.2M Potassium iodide