7E74

Crystal structure of ENL YEATS domain T3 mutant in complex with histone H3 acetylation at K27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-27
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	82.789, 91.309, 165.823
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.970 - 2.900
R-factor	0.2103
Rwork	0.208
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j9s
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.960
High resolution limit [Å]	2.900	7.890	2.910
Rmerge	0.239	0.095	0.955
Rmeas	0.248	0.099	0.992
Rpim	0.068	0.028	0.269
Number of reflections	28625	1580	1430
<I/σ(I)>	1.7
Completeness [%]	100.0	99.9	100
Redundancy	13.1	11.8	13.4
CC(1/2)		0.997	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	2M sodium formate, 0.1M sodium acetate trihydrate pH 4.6