7DZN
Crystal Structure of the cross-restricted T18A TCR and HLAB4201 bound to HIV-1 Gag TL9 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2020-11-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9790 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 96.992, 96.992, 263.839 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.700 - 2.630 |
R-factor | 0.2102 |
Rwork | 0.207 |
R-free | 0.26070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u1j 4gg6 4udt |
RMSD bond length | 0.003 |
RMSD bond angle | 0.675 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.700 | 2.722 |
High resolution limit [Å] | 2.628 | 2.628 |
Rmerge | 0.036 | 0.299 |
Number of reflections | 38478 | 3733 |
<I/σ(I)> | 14.76 | |
Completeness [%] | 100.0 | |
Redundancy | 6.5 | |
CC(1/2) | 0.999 | 0.919 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 0.1 M SPG pH 7.0, 25 % w/v PEG 1500 |