7DRK
Crystal structure of phosphatidylglycerol phosphate synthase in complex with cytidine diphosphate-diacylglycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 127.781, 59.432, 73.027 |
Unit cell angles | 90.00, 106.66, 90.00 |
Refinement procedure
Resolution | 9.990 - 3.000 |
R-factor | 0.2523 |
Rwork | 0.250 |
R-free | 0.29920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7drj |
RMSD bond length | 0.004 |
RMSD bond angle | 0.922 |
Data reduction software | XDS (Jan 31, 2020 Built=20200417) |
Data scaling software | Aimless (CCP4-7.0.078) |
Phasing software | PHASER (1.17.1_3660) |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.070 |
High resolution limit [Å] | 2.890 | 2.890 |
Rmerge | 0.174 | 1.118 |
Number of reflections | 11858 | 2344 |
<I/σ(I)> | 5.8 | 1.4 |
Completeness [%] | 99.5 | 98.7 |
Redundancy | 4.6 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.5 | 293 | 15-26% (v/v) 1,4-butanediol, 0.2M zinc acetate, 0.1M imidazole/HCl (pH 5.0-7.5) |