7DR4

Complex of anti-human IL-2 antibody and human IL-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	79.334, 72.261, 210.930
Unit cell angles	90.00, 92.71, 90.00

Refinement procedure

Resolution	29.790 - 2.490
R-factor	0.1964
Rwork	0.195
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lqb
RMSD bond length	0.008
RMSD bond angle	1.014
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.250	2.500
High resolution limit [Å]	2.490	2.500
Rmeas	0.179
Rpim	0.083
Number of reflections	80414	5025
<I/σ(I)>	1.34
Completeness [%]	96.3
Redundancy	4.3
CC(1/2)	0.980	0.442

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M NH4NO3, pH4.8, 25% PEG3350