7DIY

Crystal structure of SARS-CoV-2 nsp10 bound to nsp14-exoribonuclease domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	53.929, 68.280, 109.739
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.771 - 2.693
R-factor	0.2319
Rwork	0.230
R-free	0.26370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c8t
RMSD bond length	0.007
RMSD bond angle	0.840
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.690	2.690
Rmerge	0.128	0.453
Number of reflections	11557	1101
<I/σ(I)>	11
Completeness [%]	99.6
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1 M MOPS, 0.1 M Magnesium acetate tetrahydrate and 12 % w/v PEG 8000