7DHZ
Arsenic-bound p53 DNA-binding domain mutant R249S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.551, 68.768, 66.784 |
| Unit cell angles | 90.00, 93.22, 90.00 |
Refinement procedure
| Resolution | 41.520 - 1.740 |
| R-factor | 0.2001 |
| Rwork | 0.197 |
| R-free | 0.25650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d06 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.394 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.740 | 1.750 |
| Rmerge | 0.095 | 0.490 |
| Number of reflections | 36027 | 3578 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 94.3 | |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M Bis-Tris (pH 7) and 22% PEG5000-MME |
