7DHG

Crystal structure of SARS-CoV-2 Orf9b complex with human TOM70

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	54.655, 79.438, 124.027
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.200
R-factor	0.2263
Rwork	0.224
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kdt
RMSD bond length	0.002
RMSD bond angle	0.444
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.890	2.330
High resolution limit [Å]	2.200	2.200
Rmerge	0.099	1.350
Number of reflections	51880	7594
<I/σ(I)>	10.33	0.71
Completeness [%]	98.1	88.9
Redundancy	5.78	2.99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	296	0.1m Bis-tris ph5.5,21%PEG3350