7KDT

Human Tom70 in complex with SARS CoV2 Orf9b

Summary for 7KDT

• Entry DOI: 10.2210/pdb7kdt/pdb
• EMDB information: 22829
• Descriptor: Mitochondrial import receptor subunit TOM70, ORF9b protein (2 entities in total)
• Functional Keywords: orf9b, tom70, mitochondria, virus infection, sars cov2, viral protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 67684.36

Authors

QCRG Structural Biology Consortium (deposition date: 2020-10-09, release date: 2020-10-21, Last modification date: 2024-03-06)

Primary citation

Gordon, D.E.,Hiatt, J.,Bouhaddou, M.,Rezelj, V.V.,Ulferts, S.,Braberg, H.,Jureka, A.S.,Obernier, K.,Guo, J.Z.,Batra, J.,Kaake, R.M.,Weckstein, A.R.,Owens, T.W.,Gupta, M.,Pourmal, S.,Titus, E.W.,Cakir, M.,Soucheray, M.,McGregor, M.,Cakir, Z.,Jang, G.,O'Meara, M.J.,Tummino, T.A.,Zhang, Z.,Foussard, H.,Rojc, A.,Zhou, Y.,Kuchenov, D.,Huttenhain, R.,Xu, J.,Eckhardt, M.,Swaney, D.L.,Fabius, J.M.,Ummadi, M.,Tutuncuoglu, B.,Rathore, U.,Modak, M.,Haas, P.,Haas, K.M.,Naing, Z.Z.C.,Pulido, E.H.,Shi, Y.,Barrio-Hernandez, I.,Memon, D.,Petsalaki, E.,Dunham, A.,Marrero, M.C.,Burke, D.,Koh, C.,Vallet, T.,Silvas, J.A.,Azumaya, C.M.,Billesbolle, C.,Brilot, A.F.,Campbell, M.G.,Diallo, A.,Dickinson, M.S.,Diwanji, D.,Herrera, N.,Hoppe, N.,Kratochvil, H.T.,Liu, Y.,Merz, G.E.,Moritz, M.,Nguyen, H.C.,Nowotny, C.,Puchades, C.,Rizo, A.N.,Schulze-Gahmen, U.,Smith, A.M.,Sun, M.,Young, I.D.,Zhao, J.,Asarnow, D.,Biel, J.,Bowen, A.,Braxton, J.R.,Chen, J.,Chio, C.M.,Chio, U.S.,Deshpande, I.,Doan, L.,Faust, B.,Flores, S.,Jin, M.,Kim, K.,Lam, V.L.,Li, F.,Li, J.,Li, Y.L.,Li, Y.,Liu, X.,Lo, M.,Lopez, K.E.,Melo, A.A.,Moss 3rd, F.R.,Nguyen, P.,Paulino, J.,Pawar, K.I.,Peters, J.K.,Pospiech Jr., T.H.,Safari, M.,Sangwan, S.,Schaefer, K.,Thomas, P.V.,Thwin, A.C.,Trenker, R.,Tse, E.,Tsui, T.K.M.,Wang, F.,Whitis, N.,Yu, Z.,Zhang, K.,Zhang, Y.,Zhou, F.,Saltzberg, D.,Hodder, A.J.,Shun-Shion, A.S.,Williams, D.M.,White, K.M.,Rosales, R.,Kehrer, T.,Miorin, L.,Moreno, E.,Patel, A.H.,Rihn, S.,Khalid, M.M.,Vallejo-Gracia, A.,Fozouni, P.,Simoneau, C.R.,Roth, T.L.,Wu, D.,Karim, M.A.,Ghoussaini, M.,Dunham, I.,Berardi, F.,Weigang, S.,Chazal, M.,Park, J.,Logue, J.,McGrath, M.,Weston, S.,Haupt, R.,Hastie, C.J.,Elliott, M.,Brown, F.,Burness, K.A.,Reid, E.,Dorward, M.,Johnson, C.,Wilkinson, S.G.,Geyer, A.,Giesel, D.M.,Baillie, C.,Raggett, S.,Leech, H.,Toth, R.,Goodman, N.,Keough, K.C.,Lind, A.L.,Klesh, R.J.,Hemphill, K.R.,Carlson-Stevermer, J.,Oki, J.,Holden, K.,Maures, T.,Pollard, K.S.,Sali, A.,Agard, D.A.,Cheng, Y.,Fraser, J.S.,Frost, A.,Jura, N.,Kortemme, T.,Manglik, A.,Southworth, D.R.,Stroud, R.M.,Alessi, D.R.,Davies, P.,Frieman, M.B.,Ideker, T.,Abate, C.,Jouvenet, N.,Kochs, G.,Shoichet, B.,Ott, M.,Palmarini, M.,Shokat, K.M.,Garcia-Sastre, A.,Rassen, J.A.,Grosse, R.,Rosenberg, O.S.,Verba, K.A.,Basler, C.F.,Vignuzzi, M.,Peden, A.A.,Beltrao, P.,Krogan, N.J.
Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms.
Science, 370:-, 2020

PubMed Abstract: The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a grave threat to public health and the global economy. SARS-CoV-2 is closely related to the more lethal but less transmissible coronaviruses SARS-CoV-1 and Middle East respiratory syndrome coronavirus (MERS-CoV). Here, we have carried out comparative viral-human protein-protein interaction and viral protein localization analyses for all three viruses. Subsequent functional genetic screening identified host factors that functionally impinge on coronavirus proliferation, including Tom70, a mitochondrial chaperone protein that interacts with both SARS-CoV-1 and SARS-CoV-2 ORF9b, an interaction we structurally characterized using cryo-electron microscopy. Combining genetically validated host factors with both COVID-19 patient genetic data and medical billing records identified molecular mechanisms and potential drug treatments that merit further molecular and clinical study.

PubMed: 33060197
DOI: 10.1126/science.abe9403

Experimental method

ELECTRON MICROSCOPY (3.05 Å)