7DF1
Crystal structure of human CD98 heavy chain extracellular domain in complex with S1-F4 scFv
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2018-04-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 125.802, 165.212, 176.191 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.044 - 2.806 |
R-factor | 0.2177 |
Rwork | 0.217 |
R-free | 0.24860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dh2 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.554 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.050 | 2.880 |
High resolution limit [Å] | 2.806 | 2.810 |
Rmerge | 0.118 | |
Number of reflections | 90217 | 6299 |
<I/σ(I)> | 12.69 | |
Completeness [%] | 99.8 | |
Redundancy | 6.97 | |
CC(1/2) | 0.998 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium citrate tribasic dihydrate pH 5.0, 9% PEG20000, 5% PEG400, 9% glycerol |