7DD3
Cryo-EM structure of the pre-mRNA-loaded DEAH-box ATPase/helicase Prp2 in complex with Spp2
EM specimen
| Vitrification | Yes |
| Staining | Yes |
| Staining material | Uranyl Acetate |
EM Data Collection
| Microscope | FEI TITAN |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
