7D25
Hsp90 alpha N-terminal domain in complex with a 14 compund
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 67.604, 89.828, 98.231 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.961 - 1.650 |
R-factor | 0.1876 |
Rwork | 0.186 |
R-free | 0.21420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h22 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
Rmerge | 0.053 | 0.041 | 0.252 |
Total number of observations | 207351 | ||
Number of reflections | 35747 | 3764 | 3445 |
<I/σ(I)> | 16.1 | ||
Completeness [%] | 98.5 | 98.9 | 96.5 |
Redundancy | 5.8 | 7.5 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 1.0M ammonium Sulfate, 0.1M Tris-HCl (pH8.5) |