7CRZ

Crystal structure of human glucose transporter GLUT3 bound with C3361

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-10-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	48.329, 118.248, 54.252
Unit cell angles	90.00, 103.54, 90.00

Refinement procedure

Resolution	19.806 - 2.300
R-factor	0.168
Rwork	0.166
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zw9
RMSD bond length	0.006
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	26341	3132
<I/σ(I)>	8.57
Completeness [%]	99.9
Redundancy	99.2
CC(1/2)	0.999	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7	293	0.1 M NH4Cl, 0.1 M HEPES pH 7.0, 40% PEG 400