7CMZ

Crystal Structure of BRCT7/8 in Complex with the APS Motif of PHF8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-12-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97930
Spacegroup name	P 21 21 21
Unit cell lengths	54.517, 60.350, 66.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.520 - 1.695
R-factor	0.18
Rwork	0.178
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3al2
RMSD bond length	0.011
RMSD bond angle	1.679
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.730
High resolution limit [Å]	1.695	4.610	1.695
Rmerge	0.055	0.041	0.375
Rmeas	0.059	0.044	0.409
Rpim	0.023	0.017	0.162
Number of reflections	24740	1366	1198
<I/σ(I)>	8.5
Completeness [%]	99.9	99.6	99.9
Redundancy	6.4	6.1	6.2
CC(1/2)	0.994	0.998	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic