7CMM
Crystal structure of TEAD1-YBD in complex with K-975
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-05 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 147.990, 151.810, 67.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.500 |
R-factor | 0.2363 |
Rwork | 0.234 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kys |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.330 | 49.330 | 3.830 |
High resolution limit [Å] | 3.500 | 8.570 | 3.500 |
Rmerge | 0.546 | 0.070 | 3.290 |
Rmeas | 0.562 | 0.072 | 3.377 |
Rpim | 0.129 | 0.017 | 0.758 |
Total number of observations | 369455 | 24984 | 90791 |
Number of reflections | 19877 | 1484 | 4647 |
<I/σ(I)> | 8 | 34.7 | 1.3 |
Completeness [%] | 99.7 | 99.5 | 99.7 |
Redundancy | 18.6 | 16.8 | 19.5 |
CC(1/2) | 0.989 | 0.997 | 0.614 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | ammonium sulfate, PEG 3350, Bis-Tris |