7CHZ
Crystal Structure Of Human Il-1beta In Complex With Antibody Binding Fragment Of IgG26A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2016-12-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.694, 112.699, 38.935 |
| Unit cell angles | 90.00, 94.53, 90.00 |
Refinement procedure
| Resolution | 23.941 - 2.500 |
| Rwork | 0.193 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i1b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.580 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.570 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 23536 | 2268 |
| <I/σ(I)> | 28.8 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.880 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 17% (w/v) PEG 3350, 10% (v/v) glycerol, and 0.1 M citric acid at pH 4.0 |
