7CDD

Crystal structure of LSD1-CoREST in complex with PRSFLVRR peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-04
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	I 2 2 2
Unit cell lengths	121.987, 180.515, 233.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.760
R-factor	0.20496
Rwork	0.204
R-free	0.22733
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h6q
RMSD bond length	0.003
RMSD bond angle	1.269
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	2.820
High resolution limit [Å]	2.760	2.760
Number of reflections	66319	4405
<I/σ(I)>	16	1.7
Completeness [%]	100.0
Redundancy	13.3
CC(1/2)	0.999	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M N-(carbamoylmethyl)iminodiacetic acid, 1.23 M potassium sodium tartrate tetrahydrate