7CD5

mAPE1-blunt-ended dsDNA product complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-23
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97622
Spacegroup name	P 65 2 2
Unit cell lengths	107.210, 107.210, 230.792
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.892 - 2.700
R-factor	0.191
Rwork	0.189
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hd7
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.800
High resolution limit [Å]	2.700	5.800	2.700
Rmerge	0.091	0.044	0.499
Rmeas	0.093	0.045	0.517
Rpim	0.020	0.010	0.133
Total number of observations	431037
Number of reflections	22366	2477	2061
<I/σ(I)>	8.4
Completeness [%]	99.5	99.9	94.9
Redundancy	19.3	20.4	13.6
CC(1/2)		1.000	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M Bis-Tris pH 6.5, 25% v/v Polyethylene glycol 300