7CBY

Structure of FOXG1 DNA binding domain bound to DBE2 DNA site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	48.126, 61.446, 73.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.871 - 1.646
R-factor	0.1575
Rwork	0.156
R-free	0.18220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ako
RMSD bond length	0.009
RMSD bond angle	1.117
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.900	1.700
High resolution limit [Å]	1.640	1.640
Rmeas	0.035
Rpim		0.312
Number of reflections	26897	2635
<I/σ(I)>	20.7
Completeness [%]	98.9
Redundancy	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	80 mM NaCl, 40 mM Sodium cacodylate, pH 6.0, 45% 2-Methyl-2,4-pentanediol, 12 mM spermine