7C9O
Crystal structure of DNA-bound CCT/NF-YB/YC complex (HD1CCT/GHD8/OsNF-YC2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97914 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 136.080, 136.080, 62.960 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.020 - 2.550 |
R-factor | 0.2008 |
Rwork | 0.200 |
R-free | 0.22580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g92 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.094 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14rc3_3206) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.040 | 2.660 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.090 | 0.800 |
Rmeas | 0.094 | 0.845 |
Rpim | 0.029 | 0.267 |
Number of reflections | 22041 | 2658 |
<I/σ(I)> | 14.8 | 2.4 |
Completeness [%] | 99.5 | |
Redundancy | 9.9 | |
CC(1/2) | 0.999 | 0.865 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 4000, sodium citrate, sodium acetate trihydrate, magnesium acetate tetrahydrate, glycine |