7C9O

Crystal structure of DNA-bound CCT/NF-YB/YC complex (HD1CCT/GHD8/OsNF-YC2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97914
Spacegroup name	P 32 2 1
Unit cell lengths	136.080, 136.080, 62.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.020 - 2.550
R-factor	0.2008
Rwork	0.200
R-free	0.22580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g92
RMSD bond length	0.010
RMSD bond angle	1.094
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14rc3_3206)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.040	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.090	0.800
Rmeas	0.094	0.845
Rpim	0.029	0.267
Number of reflections	22041	2658
<I/σ(I)>	14.8	2.4
Completeness [%]	99.5
Redundancy	9.9
CC(1/2)	0.999	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 4000, sodium citrate, sodium acetate trihydrate, magnesium acetate tetrahydrate, glycine