7C6B

Crystal structure of Ago2 MID domain in complex with 6-(3-(2-carboxyethyl)phenyl)purine riboside monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	47.280, 66.460, 40.420
Unit cell angles	107.06, 95.63, 86.40

Refinement procedure

Resolution	38.510 - 1.700
R-factor	0.1962
Rwork	0.194
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6L45
RMSD bond length	0.010
RMSD bond angle	1.717
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.510	38.510	1.800
High resolution limit [Å]	1.700	5.100	1.700
Rmerge	0.191	0.126	1.100
Rmeas	0.215	0.143	1.307
Total number of observations	228418
Number of reflections	51572	1902	8073
<I/σ(I)>	5.44	8.48	1.44
Completeness [%]	99.8	99.1	99.5
Redundancy	4.429	4.632	3.351
CC(1/2)	0.982	0.986	0.601

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277.15	0.1M imidazole pH8.0, 0.2M NaCl, 0.46M NaH2PO4, 1.84M K2HPO4