7C6A
Crystal structure of AT2R-BRIL and SRP2070_Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.230, 131.040, 113.800 |
| Unit cell angles | 90.00, 97.07, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.400 |
| R-factor | 0.2378 |
| Rwork | 0.235 |
| R-free | 0.28530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zud |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.303 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.770 | 42.770 | 3.670 |
| High resolution limit [Å] | 3.400 | 9.000 | 3.400 |
| Rmerge | 0.607 | 0.184 | 2.813 |
| Rmeas | 0.623 | 0.188 | 2.907 |
| Rpim | 0.140 | 0.040 | 0.720 |
| Total number of observations | 303868 | 19100 | 48531 |
| Number of reflections | 16178 | 899 | 3344 |
| <I/σ(I)> | 5.1 | 14.3 | 1.1 |
| Completeness [%] | 100.0 | 99.1 | 100 |
| Redundancy | 18.8 | 21.2 | 14.5 |
| CC(1/2) | 0.985 | 0.991 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 0.05M POTASSIUM ACETATE, 0.1M MES PH6.5, 26-36% PEG300 |
