7C41

KRAS G12V and H-REV107 peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-27
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	41.907, 84.339, 84.335
Unit cell angles	59.98, 89.96, 89.97

Refinement procedure

Resolution	36.522 - 2.276
R-factor	0.2613
Rwork	0.260
R-free	0.28340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uqw
RMSD bond length	0.009
RMSD bond angle	1.160
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.328
High resolution limit [Å]	2.270	2.270
Rmerge	0.102	0.200
Number of reflections	45859	2465
<I/σ(I)>	16.5
Completeness [%]	97.7
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	polyethylene glycol 3350, 0.2 M potassium nitrate at pH 6.8