7BWT

SopD-Rab8 complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	114.660, 114.660, 107.066
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	57.330 - 2.300
R-factor	0.1898
Rwork	0.188
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cpc 4lhw
RMSD bond length	0.003
RMSD bond angle	0.586
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.330	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.063
Number of reflections	36383	5694
<I/σ(I)>	19.32
Completeness [%]	99.0
Redundancy	9
CC(1/2)	0.999	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.05M Sodium cacodylate trihydrate pH 6.5, 0.2 M MgCl2, 11.5% PEG4000