7BV5

Crystal structure of the yeast heterodimeric ADAT2/3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	138.591, 118.543, 91.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.2027
Rwork	0.200
R-free	0.25460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dh1
RMSD bond length	0.012
RMSD bond angle	1.562
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.205	0.818
Number of reflections	37634	3637
<I/σ(I)>	7.8	1.4
Completeness [%]	99.7	98.2
Redundancy	6.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20-25% PEG 400, 0.1 M MES (pH 6.0-6.5)