7BJY
Crystal structure of the first bromodomain (BD1) of human BRDT bound to Ro3280
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9700 |
| Spacegroup name | P 61 2 2 |
| 格子定数 [Å] | 57.040, 57.040, 407.870 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 分解能 | 42.254 - 2.220 |
| R因子 | 0.2174 |
| Rwork | 0.215 |
| R-free | 0.25550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7l73 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 42.254 | 42.254 | 2.280 |
| 分解能 [Å] (高) | 2.220 | 9.930 | 2.220 |
| Rmerge_l_obs | 0.132 | 0.030 | 1.113 |
| Rmeas | 0.135 | 0.031 | 1.141 |
| Total number of observations | 441196 | ||
| 独立反射数 | 20864 | 309 | 1501 |
| <I/σ(I)> | 19.24 | 45.17 | 3 |
| 完全性 [%] | 99.9 | 98.7 | 100 |
| 冗長性 | 21.146 | 16.249 | 20.266 |
| CC(1/2) | 1.000 | 1.000 | 0.831 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M NaCl, 0.1 M Bis-Tris pH5.5, 25% w/v PEG 3,350 |
