7BIX
Crystal structure of UMPK from M. tuberculosis in complex with UDP and UTP (C2 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 197.470, 152.820, 125.110 |
| Unit cell angles | 90.00, 121.39, 90.00 |
Refinement procedure
| Resolution | 27.980 - 3.120 |
| Rwork | 0.191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nwy |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.980 | 3.231 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Rmerge | 0.205 | 1.219 |
| Rmeas | 0.221 | 1.319 |
| Rpim | 0.082 | 0.498 |
| Number of reflections | 56229 | 3643 |
| <I/σ(I)> | 7.45 | 1.23 |
| Completeness [%] | 93.9 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.992 | 0.579 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.7 | 293 | 1 M LiCl, 20 % (w/v) PEG 8K and 0.1 M Tris pH 8.7 |
