7BBJ
CD73 in complex with the humanized antagonistic antibody mAb19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99985 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 133.350, 67.740, 150.050 |
| Unit cell angles | 90.00, 100.13, 90.00 |
Refinement procedure
| Resolution | 39.830 - 2.720 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h2f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 147.700 | 2.865 |
| High resolution limit [Å] | 2.726 | 2.726 |
| Number of reflections | 59881 | 2994 |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 88.3 | |
| Redundancy | 4.7 | |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 12 % w/v PEG 8000, 0.1 M magnesium Acetate and 0.1 M MOPS, pH 7.5 |
