7BBG

CRYSTAL STRUCTURE OF HLA-A2-WT1-RMF AND FAB 11D06

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.000010
Spacegroup name	P 1 21 1
Unit cell lengths	54.111, 67.009, 139.361
Unit cell angles	90.00, 90.57, 90.00

Refinement procedure

Resolution	48.300 - 2.640
R-factor	0.171
Rwork	0.168
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in house Fab fragment structure
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	2.770
High resolution limit [Å]	2.640	2.640
Rmerge	0.079	0.970
Number of reflections	29643	3934
<I/σ(I)>	13.1
Completeness [%]	99.8
Redundancy	3.85
CC(1/2)	0.992	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris pH 8.0, 20% PEG 4000