7BAI

Structure of RIG-I CTD (I875A) bound to p-RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9999
Spacegroup name	C 2 2 21
Unit cell lengths	84.523, 135.126, 110.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.700 - 3.400
R-factor	0.2238
Rwork	0.218
R-free	0.27330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ncu
RMSD bond length	0.004
RMSD bond angle	0.956
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.720	3.350
High resolution limit [Å]	3.300	3.300
Rmeas	0.270	1.240
Number of reflections	8984	0
<I/σ(I)>	6.3
Completeness [%]	99.7
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	0.2 M thiocyanate pH 8.4 20% PEG 3350 10% ethylene glycol 10% MPD